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We report a new record of Austrolebias vandenbergi from the Gran Chaco of southeastern Bolivia and present a distribution map with known localities for this species. We also provide information on the diet and female fecundity of these Bolivian specimens.

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First record of Van den Berg’s Pearlfish, Austrolebias vandenbergi Huber, 1995 (Cyprinodontiformes: Rivulidae) in Bolivia with comments on its diet and reproductive biology