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This study provides the first record of Physalaemus maximus in the state of Espirito Santo, southeastern Brazil. Physalaemus maximus was recorded in the Municipality of Castelo (1100 m a.s.l.), where it was observed in explosive reproduction in an Atlantic Forest remnant on 06 January 2010. The advertisement call of P. maximus consisted of a single multipulsed note, with carrier frequency emitted in 1,250 Hz and mean duration of 2.10 s. The record from Castelo extends in approximately 140 km eastward from the previous geographic distribution admitted for the species.

Physalaemus maximus Feio, Pombal Jr. and Caramaschi, 1999 (Anura: Leiuperidae): distribution extension and advertisement call