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This study reports the expansion of the known distribution of the invasive fish Xiphophorus maculatus (Gunther, 1866) into the upper Magdalena river basin. The new record comes from a wetland in the Dry Tropical Forest biome in Tolima and represents the first collection of this species from the upper Magdalena river basin. An updated distribution for Colombia is provided.

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Range extension of the invasive fish Xiphophorus maculatus (Günther, 1866) (Cyprinodontiformes: Poeciliidae) in the upper Magdalena river basin, Colombia