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Ancistrus cuiabae, an easily identified species of armored catfish from the Upper Paraguay River basin has recently been described from the Cuiabá River basin. Its distribution was previously restricted to the state of Mato Grosso. However, here we report the southernmost record of this species, the first within the Mato Grosso do Sul State.

Ancistrus cuiabae Knaack, 1999 (Siluriformes: Loricariidae: Ancistrinae): first record for the Southern Pantanal