Open AccessDevelopment of a multiplex event-specific PCR assay for detection of genetically modified rice
Author(s) -
Jingwen Lu,
G.Z. JI,
Guotong Li,
Yanfang Wu,
J. Yang,
Shu-Fang Lin,
Danhe Yang,
Jie Zhao,
Weiming Xiu
Publication year - 2015
Publication title -
cereal research communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.28
H-Index - 32
eISSN - 1788-9170
pISSN - 0133-3720
DOI - 10.1556/0806.43.2015.043
Subject(s) - multiplex polymerase chain reaction , multiplex , genetically modified organism , microbiology and biotechnology , contamination , biology , polymerase chain reaction , genetically modified rice , detection limit , food safety , genetically modified crops , food science , gene , genetics , chemistry , chromatography , transgene , ecology
Global rice supplies have been found contaminated with unapproved varieties of genetically modified (GM) rice in recent years, which has led to product recalls in several of countries. Faster and more effective detection of GM contamination can prevent adulterated food, feed and seed from being consumed and grown, minimize the potential environmental, health or economic damage. In this study, a simple, reliable and cost-effective multiplex polymerase chain reaction (PCR) assay for identifying genetic modifications of TT51-1, Kemingdao1 (KMD1) and Kefeng6 (KF6) rice was developed by using the event-specific fragment. The limit of detection (LOD) for each event in the multiplex PCR is approximately 0.1%. Developed multiplex PCR assays can provide a rapid and simultaneous detection of GM rice.\u
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