Effects of Citri Reticulatae Viride Pericarpium on 4-Hydroxynonenal-Induced Inflammation in PC12 Cells

Received: October 7, 2016 Revised: December 13, 2016 Accepted: December 13, 2016 Objectives: The purpose of this study was to observe the effects of Citri Reticulatae Viride Pericarpium (CP) on 4-Hydroxynonenal (4-HNE)-induced inflammation in PC12 cells. Methods: 4-HNE was treated in PC12 cell to cause inflammatory response, and then treated with CP water extract at 25, 50, and 100 g/ml. The phosphorylation of Jun N-terminal kinase (JNK) and the expression of NF-B in PC12 cells were determined by Western blot, respectively. Results: The phosphorylation of JNK was significantly decreased in 4-HNE-stimulated PC12 cell by the treatment of CP extract at 25 g/ml. The 4-HNE-induced expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) p65 in nuclear of the cells was significantly decreased in PC12 cell by treatment with CP extract at 25, 50, and 100 g/ml. Conclusions: These results suggest that CP water extract has an anti-inflammatory activity through suppressing the JNK and NF-B activation.