Валідація методу кількісного визначення нітрофурану (АОЗ) і хлорамфеніколу у зразках меду натурального за допомогою тест-системи RidaScreen®

We presented the validation method of the quantitative determination of Nitrofuran (AOZ) and Chloramphenicol in honey samples by the RidaScreen® test system (according to Commission Decision 2002/657/EC and Guidelines for the validation of screening methods for residues of veterinary medicines (initial validation and transfer) of 20/01/2010). We revealed several validation indicators like ccβ (detection capability), accuracy (precision), recovery and some others during the initial validation (specificity and selectivity). We determined the minimum detection values of Nitrofuran (AOZ) in honey, the highest value (the highest response) for blank samples is 0.066 μg/kg and the lowest value for enriched samples (lowest response) is 0.401 μg/kg. Based on the obtained results none of the responses for enriched samples do not coincide with the range of replies of blank samples. Hence, it follows that the detection capability (CCβ) for this screening method is less than or equal to 0.4 μg/kg. For the determination of Chloramphenicol, the highest response is noted for blank samples – 0.021 μg/kg (21 ng/kg) and the lowest response noted for enriched samples is 0.048 μg/kg (48 ng/kg). None of the responses for enriched samples do not coincide with the range of replies of blank matrix (samples), therefore we could conclude that the CCβ of this screening method is less than or equal to 0.05 μg/kg (β-error < 5 %) and the cut-off value of this test is 0.048. According to the validation the lowest value of substances that could detected by ELISA using the following test systems for competitive ELISA (enzyme-linked immunoassay) for Nitrofuran (AOZ) – 0.4 μg/kg, for Chloramphenicol – 0.05 μg/kg. Described in State Standard 4497:2005 “Natural Honey. Technical conditions” the maximum allowable level for Chloramphenicol – 0.3 μg/kg and Nitrofuran (AOZ) – 0.6 μg/kg require correction according to the established validation parameters.