IN VITRO BIOSYNTHESIS AND ANTIMICROBIAL POTENTIAL OF BIOLOGICALLY REDUCED GRAPHENE OXIDE/AG NANOCOMPOSITE AT ROOM TEMPERATURE | Zendy

Mohamed M. ElZahed | Zendy; Zakaria Baka | Zendy; Mohamed Abou-Dobara | Zendy; Ahmed El-Sayed | Zendy

Open Access

IN VITRO BIOSYNTHESIS AND ANTIMICROBIAL POTENTIAL OF BIOLOGICALLY REDUCED GRAPHENE OXIDE/AG NANOCOMPOSITE AT ROOM TEMPERATURE

Author(s) -

Mohamed M. ElZahed,

Zakaria Baka,

Mohamed Abou-Dobara,

Ahmed El-Sayed

Publication year - 2021

Publication title -

journal of microbiology biotechnology and food sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.186

H-Index - 7

ISSN - 1338-5178

DOI - 10.15414/jmbfs.3956

Subject(s) - antimicrobial , graphene , bacillus cereus , candida albicans , escherichia coli , fourier transform infrared spectroscopy , klebsiella pneumoniae , yeast , microbiology and biotechnology , nanocomposite , chemistry , staphylococcus aureus , bacteria , nuclear chemistry , materials science , biology , nanotechnology , biochemistry , chemical engineering , genetics , engineering , gene

Antimicrobial resistance is one of the most serious problems that continues to challenge the public health threats and agriculture sectors. The study highlighted one-step method for reduced graphene oxide/Ag nanocomposite (rGO/AgNC) biosynthesis using the supernatant of Escherichia coli D8 (MF062579) strain at room temperature and sunlight. The rGO/AgNC was characterized by UV-vis spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). Results showed those the rGO-annealed AgNPs showed absorption peak at 430 nm and have been obtained with an average particle size of 8-17±9.1 nm. The MIC value of rGO/AgNC (6.25 μg/mL) showed in vitro antimicrobial inhibition against pathogenic bacterial strains such as Gram-negative bacteria (E. coli ATCC25922; 79%, Klebsiella pneumoniae ATCC33495; 87%) and Gram-positive bacteria (Staphylococcus aureus ATCC25923; 91% and Bacillus cereus ATCC6633; 53%) as well as Candida albicans ATCC10231; 60% as pathogenic yeast.

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