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Antimicrobial resistance is one of the most serious problems that continues to challenge the public health threats and agriculture sectors. The study highlighted one-step method for reduced graphene oxide/Ag nanocomposite (rGO/AgNC) biosynthesis using the supernatant of Escherichia coli D8 (MF062579) strain at room temperature and sunlight. The rGO/AgNC was characterized by UV-vis spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). Results showed those the rGO-annealed AgNPs showed absorption peak at 430 nm and have been obtained with an average particle size of 8-17±9.1 nm. The MIC value of rGO/AgNC (6.25 μg/mL) showed in vitro antimicrobial inhibition against pathogenic bacterial strains such as Gram-negative bacteria (E. coli ATCC25922; 79%, Klebsiella pneumoniae ATCC33495; 87%) and Gram-positive bacteria (Staphylococcus aureus ATCC25923; 91% and Bacillus cereus ATCC6633; 53%) as well as Candida albicans ATCC10231; 60% as pathogenic yeast.

IN VITRO BIOSYNTHESIS AND ANTIMICROBIAL POTENTIAL OF BIOLOGICALLY REDUCED GRAPHENE OXIDE/AG NANOCOMPOSITE AT ROOM TEMPERATURE