THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES

87 There are great achievements in the study of electrophysical characteristics of the different parts of brain at biotechnology development [1, 2], which can be compared with details of its morphological structure determined by magnetic resonance tomography (MRT) [3, 4]. It is known, there is 84% of intracellular water in brain (in neurons and glia). The presence of so much water amount in brain suggests the possibility of its active participation in the brain function not only as a medium providing delivery of nutrients to the cells and removing decomposition products (it is carried out by neuroglia cells) but also in specific functions performing. The water in brain tissue cells is studied by NMR spectroscopy for more than 50 years [6– 9] which served largely as the basis for creation and wide spreading of MRT methods. As a basic research method the low-temperature Н NMR spectroscopy was chosen [10–13], whereby we can determine the amount of strongly and weakly bound water by changes in the intensity of the NMR signal during the thawing process of samples, and using Gibbs-Thomson equations — the nonfreezing water clusters radii distribution [14, 15]. The magnitude of water chemical shift made it possible to calculate the average association degree of water molecules in polyassociates. This takes into account the fact that protons of not associated (weakly associated) water have a chemical shift Н = 1–1.5 ppm, icelike structures characteristic for hexagonal ice have Н = 7 ppm [16], and liquid water has Н = 4.5–5 ppm. UDK 544.723:612.82:616.831-005.1-06-07 doi: 10.15407/biotech8.06.087