ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION

63 Current methods of low-temperature storage of packed white cells cannot guarantee their high safety, which is due to heterogeneity of cell population in terms of composition and functional characteristics and, therefore, difficulty of unification of optimal cryopreservation conditions. The experimental research conducted to date both in Ukraine and abroad aimed at finding of better methods of leukocytes cryopreservation [1–5], which requires separation of packed white cells into individual populations and are not always clinically justified. Despite some progress, frozen-thawed leukocytes need to recovery of their functional status before clinical application. It is known that in leukocytes intensity bioenergetic processes changes after cryopreservation, it leads to abnormalities in energy-dependent reactions [6, 7] and hence to impairment of their functional activity. Recovery from cryoinjuries, first of all, requires energy costs, which can be replenished via glycolysis and oxidative phosphorylation. To solve this problem, use of special rehabilitating media capable of stimulating energy generation processes is promising. We present the results of studying energy metabolism of donor blood packed white cells cryopreserved with dimethylacetamide. Evidence of a stimulating effect of a lowmolecular fraction from cow cord blood (CBF) on ATP, ADP and AMP contents and glycolysis in the test cells is adduced.