APPLICATION OF IMMUNOGLOBULIN-BINDING PROTEINS A, G, L IN THE AFFINITY CHROMATOGRAPHY

BIOTECHNOLOGIA ACTA, V. 7, No 2, 2014Nowadays antibodies are widely used in the medical biotechnology and fundamental research, purity level of antibodies has a great importance for their application. Combinations of separate methods, particularly, ethanol precipitation and different methods of chromatography (ion-exchange, hydrophobic interactions and metal affinity) are used for obtaining of great amounts of antibodies with high level of purity [1]. But these methods are nonspecific, application of them leads to the particular loss of antibodies functional activity. High productivity way for antibodies obtaining is affinity chromatography with application of sorbents on the basis of immobilized immunoglobulin binding proteins, particularly, recombinant protein A