IMPACT OF Pleurotus tuber-regium (Rumph. ex Fr.) EXTRACT-LOADED NANOPARTICLES ON THYROID PROFILE OF RATS

Aim. The aim of this study was to investigate the impact of Pleurotus tuber-regium extract loaded silver nanoparticles (SNPs) on thyroid profile of rats. Methods. Acute toxicity test was performed following up and down procedure and the analysis of thyroid profile was performed following chemiluminescence assay method. Results. Acute toxicity test showed no mortality and no behavioral changes in rats treated with 2000 mg kg-1 of SNPs. High dose (400 mg kg-1) of SNPs showed high hyper thyroid activity by increasing T3 = 38.20±4.06 ng dL-1; T4 = 4.40±0.32µg dL-1; and by decreasing TSH = 0.73±0.06µIU mL-1 compared to control group (T3 = 30.20±1.02 ng dL-1; T4 = 2.94±0.08µg dL-1; TSH = 0.87±0.01µIU mL-1) and animal group treated with 200 mg kg-1 dose of SNPs (T3 = 35.69±1.13 ng dL-1; T4 = 3.82±0.24µg dL-1; TSH = 0.80±0.02µIU mL-1). Conclusions. Pleurous tuber-regium extract loaded silver nanoparticles are non toxic and can be used in drug delivery as well as in the formulation of drugs used in hypothyroidism.