Open AccessOne-Hybrid Screens at the Saccharomyces cerevisiae HMR Locus Identify Novel Transcriptional Silencing Factors
Author(s) -
Erik D. Andrulis,
David C. Zappulla,
Krassimira Alexieva-Botcheva,
Carlos Evangelista,
Rolf Sternglanz
Publication year - 2004
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1534/genetics.166.1.631
Subject(s) - biology , saccharomyces cerevisiae , genetics , locus (genetics) , gene silencing , gene , computational biology
In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus.
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