CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans | Zendy

Selma Waaijers | Zendy; Vincent Portegijs | Zendy; Jana Kerver | Zendy; Bennie Lemmens | Zendy; Marcel Tijsterman | Zendy; Sander van den Heuvel | Zendy; Mike Boxem | Zendy

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CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans

Author(s) -

Selma Waaijers,

Vincent Portegijs,

Jana Kerver,

Bennie Lemmens,

Marcel Tijsterman,

Sander van den Heuvel,

Mike Boxem

Publication year - 2013

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.113.156299

Subject(s) - caenorhabditis elegans , biology , crispr , cas9 , genetics , mutagenesis , gene , genetic screen , genome , genome editing , mutation , mutant , caenorhabditis , computational biology

The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.

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