Open AccessTransgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas
Author(s) -
Hui Chiu,
H SCHWARTZ,
Igor Antoshechkin,
Paul W. Sternberg
Publication year - 2013
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1534/genetics.113.155879
Subject(s) - biology , crispr , genome editing , caenorhabditis elegans , genetics , genome , cas9 , caenorhabditis , gene , transgene , guide rna , rna , computational biology
CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.
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