Open AccessTargeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans
Author(s) -
Iskra Katic,
Helge Großhans
Publication year - 2013
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1534/genetics.113.155754
Subject(s) - caenorhabditis elegans , biology , crispr , genetics , mutation , cas9 , gene , caenorhabditis , gene conversion , allele
We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion.
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