Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila | Zendy

Zhongsheng Yu | Zendy; Mengda Ren | Zendy; Zhanxiang Wang | Zendy; Bo Zhang | Zendy; Yikang S. Rong | Zendy; Renjie Jiao | Zendy; Guanjun Gao | Zendy

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Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila

Author(s) -

Zhongsheng Yu,

Mengda Ren,

Zhanxiang Wang,

Bo Zhang,

Yikang S. Rong,

Renjie Jiao,

Guanjun Gao

Publication year - 2013

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.113.153825

Subject(s) - biology , euchromatin , crispr , genetics , cas9 , drosophila melanogaster , drosophila (subgenus) , genome editing , germline , gene , heterochromatin , genome , guide rna , crispr interference , computational biology , chromatin

We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

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