SNP-Ratio Mapping (SRM): Identifying Lethal Alleles and Mutations in Complex Genetic Backgrounds by Next-Generation Sequencing | Zendy

Heike Lindner | Zendy; Michael T. Raissig | Zendy; Christian Sailer | Zendy; Hiroko Shimosato-Asano | Zendy; Rémy Bruggmann | Zendy; Ueli Grossniklaus | Zendy

Open Access

SNP-Ratio Mapping (SRM): Identifying Lethal Alleles and Mutations in Complex Genetic Backgrounds by Next-Generation Sequencing

Author(s) -

Heike Lindner,

Michael T. Raissig,

Christian Sailer,

Hiroko Shimosato-Asano,

Rémy Bruggmann,

Ueli Grossniklaus

Publication year - 2012

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.112.141341

Subject(s) - biology , genetics , allele , snp , dna sequencing , identification (biology) , cloning (programming) , genome , positional cloning , computational biology , backcrossing , mutation , gene , genotype , single nucleotide polymorphism , phenotype , programming language , botany , computer science

We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.

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