Amber Mutants of Bacteriophage T4D: Their Isolation and Genetic Characterization | Zendy

Richard H. Epstein | Zendy; A. Bolle | Zendy; Charles Steinberg | Zendy

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Amber Mutants of Bacteriophage T4D: Their Isolation and Genetic Characterization

Author(s) -

Richard H. Epstein,

A. Bolle,

Charles Steinberg

Publication year - 2012

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.112.138438

Subject(s) - complementation , mutant , bacteriophage , biology , genetics , escherichia coli , mutation , strain (injury) , gene , genome , lysogenic cycle , anatomy

We have isolated a large number of mutants of bacteriophage T4D that are unable to form plaques on strain B of Escherichia coli, but are able to grow (nearly) normally on some other strains of E. coli, in particular strain CR63. These mutants, designated amber (am), have been characterized by complementation tests, by genetic crosses, and by their response to chemical mutagens. It is concluded that a particular subclass of base substitution mutations may give rise to amber mutants and that such mutants occur in many genes, which are widely distributed over the T4 genome.

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