Open AccessHaploidization inSaccharomyces cerevisiaeInduced by a Deficiency in Homologous Recombination
Author(s) -
Wei Song,
Thomas D. Petes
Publication year - 2012
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1534/genetics.111.138180
Subject(s) - biology , ploidy , homologous recombination , meiosis , homologous chromosome , genetics , saccharomyces cerevisiae , mating of yeast , genetic recombination , mating type , recombination , chromosome , rad52 , gene , flp frt recombination , mitotic crossover , rad51
Diploid Saccharomyes cerevisae strains lacking the RAD52 gene required for homologous recombination have a very high rate of chromosome loss. Two of four isolates subcultured ∼20 times (∼500 cell divisions) became haploid. These strains were capable of mating with wild-type haploids to produce diploid progeny capable of undergoing meiosis to produce four viable spores.
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