Successive and Targeted DNA Integrations in the Drosophila Genome by Bxb1 and φC31 Integrases | Zendy

Juan Huang | Zendy; Pallavi Ghosh | Zendy; Graham F. Hatfull | Zendy; Yang Hong | Zendy

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Successive and Targeted DNA Integrations in the Drosophila Genome by Bxb1 and φC31 Integrases

Author(s) -

Juan Huang,

Pallavi Ghosh,

Graham F. Hatfull,

Yang Hong

Publication year - 2011

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.111.129247

Subject(s) - integrases , biology , genetics , integrase , genome , dna , genome engineering , computational biology , gene , genome editing

At present ϕC31 is the only phage integrase system available for directionally regulated site-specific DNA integration in the Drosophila genome. Here we report that mycobacteriophage Bxb1 integrase also mediates targeted DNA integration in Drosophila with high specificity and efficiency. By alternately using Bxb1 and ϕC31, we were able to carry out multiple rounds of successive and targeted DNA integrations in our genomic engineering founder lines for the purpose of generating complex knock-in alleles.

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