Open AccessTissue Specificity of Caenorhabditis elegans Enhanced RNA Interference Mutants
Author(s) -
Jimmy J. Zhuang,
Craig P. Hunter
Publication year - 2011
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1534/genetics.111.127209
Subject(s) - rna interference , caenorhabditis elegans , biology , rna silencing , gene knockdown , mutant , rna , gene , genetics , caenorhabditis , microbiology and biotechnology
Gene knockdown by RNA interference (RNAi) in Caenorhabditis elegans is readily achieved by feeding bacteria expressing double-stranded RNA (dsRNA). Enhanced RNAi (Eri) mutants facilitate RNAi due to their hypersensitivity to dsRNA. Here, we compare eight Eri mutants for sensitivity to ingested dsRNA, targeting a variety of tissue-specific genes.
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