Targeting DNA Double-Strand Breaks with TAL Effector Nucleases | Zendy

Michelle Christian | Zendy; Tomáš Čermák | Zendy; Erin Doyle | Zendy; Clarice Schmidt | Zendy; Feng Zhang | Zendy; Aaron W. Hummel | Zendy; Adam J. Bogdanove | Zendy; Daniel F. Voytas | Zendy

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Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

Author(s) -

Michelle Christian,

Tomáš Čermák,

Erin Doyle,

Clarice Schmidt,

Feng Zhang,

Aaron W. Hummel,

Adam J. Bogdanove,

Daniel F. Voytas

Publication year - 2010

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.110.120717

Subject(s) - transcription activator like effector nuclease , biology , nuclease , genome editing , effector , dna , cleave , genetics , endonuclease , mutagenesis , genome engineering , computational biology , crispr , microbiology and biotechnology , gene , mutation

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.

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