Analysis of Ipl1-Mediated Phosphorylation of the Ndc80 Kinetochore Protein in Saccharomyces cerevisiae | Zendy

Bungo Akiyoshi | Zendy; Christian R. Nelson | Zendy; Jeffrey A. Ranish | Zendy; Sue Biggins | Zendy

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Analysis of Ipl1-Mediated Phosphorylation of the Ndc80 Kinetochore Protein in Saccharomyces cerevisiae

Author(s) -

Bungo Akiyoshi,

Christian R. Nelson,

Jeffrey A. Ranish,

Sue Biggins

Publication year - 2009

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.109.109041

Subject(s) - kinetochore , biology , phosphorylation , aurora b kinase , microbiology and biotechnology , spindle checkpoint , saccharomyces cerevisiae , genetics , gene , chromosome

Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint.

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