Removal of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null Mutations in Drosophila | Zendy

Alice Witsell | Zendy; Daniel P. Kane | Zendy; Sarah Rubin | Zendy; Mitch McVey | Zendy

Open Access

Removal of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null Mutations in Drosophila

Author(s) -

Alice Witsell,

Daniel P. Kane,

Sarah Rubin,

Mitch McVey

Publication year - 2009

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.109.108472

Subject(s) - biology , drosophila melanogaster , genetics , transposable element , gene , p element , drosophila (subgenus) , nucleotide excision repair , mutant , dna repair

Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.

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