A Genetic Test for Yeast Two-Hybrid Bait Competency Using RanBPM | Zendy

Chandra L. Tucker | Zendy; Leslie A Peteya | Zendy; Adrianne M. C. Pittman | Zendy; Jing Zhong | Zendy

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A Genetic Test for Yeast Two-Hybrid Bait Competency Using RanBPM

Author(s) -

Chandra L. Tucker,

Leslie A Peteya,

Adrianne M. C. Pittman,

Jing Zhong

Publication year - 2009

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.109.103069

Subject(s) - biology , yeast , genetics , saccharomyces cerevisiae , two hybrid screening , test (biology) , identification (biology) , computational biology , blot , simple (philosophy) , gene , paleontology , botany , philosophy , epistemology

We describe a simple genetic test for assessing the competency of Gal4-based baits prior to a yeast two-hybrid screen, which allows determination of whether a bait protein is expressed appropriately for an interaction to be detected. The novel test, based on interaction with the protein RanBPM, is easier and more predictive than other methods such as Western blotting, allowing identification of approximately 80% of incompetent baits prior to screening.

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