De Novo Identification of Single Nucleotide Mutations in Caenorhabditis elegans Using Array Comparative Genomic Hybridization | Zendy

Jason S Maydan | Zendy; Hatsumi Okada | Zendy; Stéphane Flibotte | Zendy; Mark L. Edgley | Zendy; Donald G. Moerman | Zendy

Open Access

De Novo Identification of Single Nucleotide Mutations in Caenorhabditis elegans Using Array Comparative Genomic Hybridization

Author(s) -

Jason S Maydan,

Hatsumi Okada,

Stéphane Flibotte,

Mark L. Edgley,

Donald G. Moerman

Publication year - 2009

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.108.100065

Subject(s) - biology , genetics , comparative genomic hybridization , genome , caenorhabditis elegans , dna microarray , single nucleotide polymorphism , copy number variation , oligonucleotide , computational biology , gene , genotype , gene expression

Array comparative genomic hybridization (aCGH) has been used primarily to detect copy-number variants between two genomes. Here we report using aCGH to detect single nucleotide mutations on oligonucleotide microarrays with overlapping 50-mer probes. This technique represents a powerful method for rapidly detecting novel homozygous single nucleotide mutations in any organism with a sequenced reference genome.

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