Open AccessUnexpected Expression Pattern of Tetracycline-Regulated Transgenes in Mice
Author(s) -
M Bao-Cutrona,
Pedro Moral
Publication year - 2009
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1534/genetics.108.097600
Subject(s) - biology , genetics , transgene , tetracycline , expression (computer science) , gene , antibiotics , computer science , programming language
In generating a conditional transgenic murine model based on a tetracycline-regulated system, we obtained unexpected patterns of expression due to the transcriptional inactivity of the tet-responder promoter. Here we show strong cell-type-restricted expression that was variegated to an extent determined by the number of responder transgene copies integrated into the host genome.
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