A Simple Polymerase Chain Reaction-Based Method for the Construction of Recombinase-Mediated Cassette Exchange Donor Vectors | Zendy

Jack R Bateman | Zendy; C-ting Wu | Zendy

Open Access

A Simple Polymerase Chain Reaction-Based Method for the Construction of Recombinase-Mediated Cassette Exchange Donor Vectors

Author(s) -

Jack R Bateman,

C-ting Wu

Publication year - 2008

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.108.094508

Subject(s) - biology , integrase , recombinase , genetics , computational biology , integrases , polymerase chain reaction , cre recombinase , simple (philosophy) , sequence (biology) , transgene , microbiology and biotechnology , recombination , dna , gene , genetically modified mouse , philosophy , epistemology

Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.

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