A New Purple Fluorescent Color Marker for Genetic Studies inSaccharomyces cerevisiaeandCandida albicans
Author(s) -
Sabine KepplerRoss,
Christine Noffz,
Neta Dean
Publication year - 2008
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1534/genetics.108.087080
Subject(s) - candida albicans , saccharomyces cerevisiae , biology , plasmid , yeast , fluorescence , genetics , saccharomyces , genetic screen , fluorescence microscope , gene , phenotype , physics , quantum mechanics
The ability to visualize cellular events by linking them to color or fluorescence changes has been an invaluable tool for biology. We describe a novel plasmid-borne color marker whose expression in yeast leads to purple-colored cells that are also brightly fluorescent. This dominant marker provides a useful tool for rapidly screening plasmid maintenance using a visual or fluorescence assay in both Saccharomyces cerevisiae and Candida albicans.
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