Independent Effects of cis- and trans-regulatory Variation on Gene Expression in Drosophila melanogaster | Zendy

Patricia J. Wittkopp | Zendy; Belinda K. Haerum | Zendy; Andrew G. Clark | Zendy

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Independent Effects of cis- and trans-regulatory Variation on Gene Expression in Drosophila melanogaster

Author(s) -

Patricia J. Wittkopp,

Belinda K. Haerum,

Andrew G. Clark

Publication year - 2008

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.107.082032

Subject(s) - biology , genetics , trans acting , gene , drosophila melanogaster , regulatory sequence , allele , regulator gene , genetic variation , regulation of gene expression , copy number variation , variation (astronomy) , genome , mutant , physics , astrophysics

Biochemical interactions between cis-regulatory DNA sequences and trans-regulatory gene products suggest that cis- and trans-acting polymorphisms may interact genetically. Here we present a strategy to test this hypothesis by comparing the relative cis-regulatory activity of two alleles in different genetic backgrounds. Of the eight genes surveyed in this study, five were affected by trans-acting variation that altered total transcript levels, two of which were also affected by differences in cis-regulation. The presence of trans-acting variation had no effect on relative cis-regulatory activity, showing that cis-regulatory polymorphisms can function independently of trans-regulatory variation. The frequency of such independent interactions on a genomic scale is yet to be determined.

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