The Hermes Transposon ofMusca domesticaIs an Efficient Tool for the Mutagenesis ofSchizosaccharomyces pombe | Zendy

Adam G. Evertts | Zendy; Christopher Plymire | Zendy; Nancy L. Craig | Zendy; Henry L. Levin | Zendy

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The Hermes Transposon ofMusca domesticaIs an Efficient Tool for the Mutagenesis ofSchizosaccharomyces pombe

Author(s) -

Adam G. Evertts,

Christopher Plymire,

Nancy L. Craig,

Henry L. Levin

Publication year - 2007

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.107.081075

Subject(s) - schizosaccharomyces pombe , housefly , biology , transposable element , musca , transposon mutagenesis , mutagenesis , genetics , schizosaccharomyces , transposase , mutation , gene , mutant , botany , larva

Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.

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