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Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.

The Hermes Transposon ofMusca domesticaIs an Efficient Tool for the Mutagenesis ofSchizosaccharomyces pombe