Inorganic Phosphate Deprivation Causes tRNA Nuclear Accumulation via Retrograde Transport inSaccharomyces cerevisiae | Zendy

Rebecca L. Hurto | Zendy; Amy H.Y. Tong | Zendy; Charles Boone | Zendy; Anita K. Hopper | Zendy

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Inorganic Phosphate Deprivation Causes tRNA Nuclear Accumulation via Retrograde Transport inSaccharomyces cerevisiae

Author(s) -

Rebecca L. Hurto,

Amy H.Y. Tong,

Charles Boone,

Anita K. Hopper

Publication year - 2007

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.106.069732

Subject(s) - transfer rna , saccharomyces cerevisiae , cytosol , biology , nuclear transport , cytoplasm , biochemistry , cell nucleus , nucleus , microbiology and biotechnology , yeast , rna , gene , genetics , enzyme

Nuclear export of tRNA is an essential eukaryotic function, yet the one known yeast tRNA nuclear exporter, Los1, is nonessential. Moreover recent studies have shown that tRNAs can move retrograde from the cytosol to the nucleus by an undefined process. Therefore, additional gene products involved in tRNA nucleus-cytosol dynamics have yet to be identified. Synthetic genetic array (SGA) analysis was employed to identify proteins involved in Los1-independent tRNA transport and in regulating tRNA nucleus-cytosol distribution. These studies uncovered synthetic interactions between los1Delta and pho88Delta involved in inorganic phopsphate uptake. Further analysis revealed that inorganic phosphate deprivation causes transient, temperature-dependent nuclear accumulation of mature cytoplasmic tRNA within nuclei via a Mtr10- and retrograde-dependent pathway, providing a novel connection between tRNA subcellular dynamics and phosphate availability.

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