Precise Centromere Mapping Using a Combination of Repeat Junction Markers and Chromatin Immunoprecipitation–Polymerase Chain Reaction | Zendy

Amy C Luce | Zendy; Anupma Sharma | Zendy; Oliver S B Mollere | Zendy; Thomas Wolfgruber | Zendy; Kiyotaka Nagaki | Zendy; Jiming Jiang | Zendy; Gernot G. Presting | Zendy; R. Kelly Dawe | Zendy

Open Access

Precise Centromere Mapping Using a Combination of Repeat Junction Markers and Chromatin Immunoprecipitation–Polymerase Chain Reaction

Author(s) -

Amy C Luce,

Anupma Sharma,

Oliver S B Mollere,

Thomas Wolfgruber,

Kiyotaka Nagaki,

Jiming Jiang,

Gernot G. Presting,

R. Kelly Dawe

Publication year - 2006

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.106.060467

Subject(s) - centromere , biology , chromatin immunoprecipitation , heterochromatin , genetics , chromatin , computational biology , position effect , recombinant dna , chromosome , gene , gene expression , promoter

Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

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