Exonuclease Requirements for Recombination of λ-Phage inrecDMutants ofEscherichia coli | Zendy

Damir Đermić | Zendy; Davor Zahradka | Zendy; Mirjana Petranović | Zendy

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Exonuclease Requirements for Recombination of λ-Phage inrecDMutants ofEscherichia coli

Author(s) -

Damir Đermić,

Davor Zahradka,

Mirjana Petranović

Publication year - 2006

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.106.060426

Subject(s) - exonuclease , biology , recbcd , escherichia coli , recombination , mutant , genetics , genetic recombination , homologous recombination , exonuclease iii , flp frt recombination , microbiology and biotechnology , dna , gene , dna polymerase

Recombination of lambda red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5'-3' exonuclease activity of RecJ and exonuclease VII is essential for lambda-recombination, whereas exonucleases of 3'-5' polarity are dispensable. The implications of the presented data on current models for recombination initiation in E. coli are discussed.

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