Evidence of Meiotic Crossover Control in Saccharomyces cerevisiae Through Mec1-Mediated Phosphorylation of Replication Protein A | Zendy

Amy J. Bartrand | Zendy; Dagmawi Iyasu | Zendy; Suzanne M. Marinco | Zendy; George S. Brush | Zendy

Open Access

Evidence of Meiotic Crossover Control in Saccharomyces cerevisiae Through Mec1-Mediated Phosphorylation of Replication Protein A

Author(s) -

Amy J. Bartrand,

Dagmawi Iyasu,

Suzanne M. Marinco,

George S. Brush

Publication year - 2005

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.105.047845

Subject(s) - replication protein a , biology , replication factor c , dna replication , origin recognition complex , phosphorylation , control of chromosome duplication , microbiology and biotechnology , ter protein , dna binding protein , genetics , eukaryotic dna replication , dna , gene , transcription factor

Replication protein A (RPA) is the major single-stranded DNA-binding protein in eukaryotes, essential for DNA replication, repair, and recombination. During mitosis and meiosis in budding yeast, RPA becomes phosphorylated in reactions that require the Mec1 protein kinase, a central checkpoint regulator and homolog of human ATR. Through mass spectrometry and site-directed mutagenesis, we have now identified a single serine residue in the middle subunit of the RPA heterotrimer that is targeted for phosphorylation by Mec1 both in vivo and in vitro. Cells containing a phosphomimetic version of RPA generated by mutation of this serine to aspartate exhibit a significant alteration in the pattern of meiotic crossovers for specific genetic intervals. These results suggest a new function of Mec1 that operates through RPA to locally control reciprocal recombination.

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