Eliminating Gene Conversion Improves High-Throughput Genetics in Saccharomyces cerevisiae | Zendy

Jewel A Daniel | Zendy; Jiyoun Yoo | Zendy; Blaine T. Bettinger | Zendy; David C. Amberg | Zendy; Daniel J. Burke | Zendy

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Eliminating Gene Conversion Improves High-Throughput Genetics in Saccharomyces cerevisiae

Author(s) -

Jewel A Daniel,

Jiyoun Yoo,

Blaine T. Bettinger,

David C. Amberg,

Daniel J. Burke

Publication year - 2005

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1534/genetics.105.047662

Subject(s) - gene conversion , schizosaccharomyces pombe , saccharomyces cerevisiae , biology , genetics , gene , reporter gene , gene expression , recombination

Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3Delta1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5+ gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.

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