Microarray Comparison of Anterior and PosteriorDrosophilaWing Imaginal Disc Cells Identifies Novel Wing Genes | Zendy

Daniel M. Ibrahim | Zendy; Brian Biehs | Zendy; Thomas B. Kornberg | Zendy; Ansgar Klebes | Zendy

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Microarray Comparison of Anterior and PosteriorDrosophilaWing Imaginal Disc Cells Identifies Novel Wing Genes

Author(s) -

Daniel M. Ibrahim,

Brian Biehs,

Thomas B. Kornberg,

Ansgar Klebes

Publication year - 2013

Publication title -

g3 genes genomes genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.468

H-Index - 66

ISSN - 2160-1836

DOI - 10.1534/g3.113.006569

Subject(s) - engrailed , imaginal disc , biology , hedgehog , wing , microbiology and biotechnology , primordium , homeobox , compartment (ship) , gene , transcription factor , hedgehog signaling pathway , decapentaplegic , bristle , rna interference , genetics , signal transduction , drosophila melanogaster , rna , oceanography , brush , electrical engineering , geology , engineering , aerospace engineering

Signaling between cells in the anterior (A) and posterior (P) compartments directs Drosophila wing disc development and is dependent on expression of the homeodomain transcription factor Engrailed (En) in P cells. Downstream of en, posteriorly expressed Hedgehog (Hh) protein signals across the A/P border to establish a developmental organizer that directs pattern formation and growth throughout the wing primordium. Here we extend investigations of the processes downstream of en by using expression array analysis to compare A and P cells. A total of 102 candidate genes were identified that express differentially in the A and P compartments; four were characterized: Stubble (Sb) expression is restricted to A cells due to repression by en. CG15905, CG16884; CG10200/hase und igel (hui) are expressed in A cells downstream of Hh signaling; and RNA interference for hui, Stubble, and CG16884 revealed that each is essential to wing development.

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