Double-Strand Break Repair and Holliday Junction Processing Are Required for Chromosome Processing in Stationary-Phase Escherichia coli Cells | Zendy

Ashley B. Williams | Zendy; Kyle M. Hetrick | Zendy; Patricia L. Foster | Zendy

Open Access

Double-Strand Break Repair and Holliday Junction Processing Are Required for Chromosome Processing in Stationary-Phase Escherichia coli Cells

Author(s) -

Ashley B. Williams,

Kyle M. Hetrick,

Patricia L. Foster

Publication year - 2011

Publication title -

g3 genes genomes genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.468

H-Index - 66

ISSN - 2160-1836

DOI - 10.1534/g3.111.001057

Subject(s) - holliday junction , recbcd , biology , chromosome , dna , microbiology and biotechnology , cell division , chromosome segregation , chromothripsis , genetics , dna repair , cell , gene , genome instability , dna damage

As nutrients are depleted and cell division ceases in batch cultures of bacteria, active processes are required to ensure that each cell has a complete copy of its genome. How chromosome number is manipulated and maintained in nondividing bacterial cells is not fully understood. Using flow cytometric analysis of cells from different growth phases, we show that the Holliday junction-processing enzymes RuvABC and RecG, as well as RecBCD, the enzyme complex that initiates DNA double-strand break repair, are required to establish the normal distribution of fluorescent peaks, which is commonly accepted to reflect the distribution of chromosome numbers. Our results reveal that these proteins are required for the proper processing of chromosomes in stationary phase.

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