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Grape ( Vitis vinifera  L.) seeds from 3 grape varieties were powdered and the fatty material was extracted. These extracts were tested for grape storage restriction fungi  Botrytis cinerea, Alternaria alternata ,  Aspregillus niger  and Penicillium expansum  and antibacteria activity for Gram-negative  Escherichia coli ,  Pseudomonas aeruginosa ,  Klebsiella pneumonia , and Gram-positive  Enterococcus faecalis ,  Streptococcus pneumonia  and  Staphylococcus aureus by pour plate method. The grape seed extracts (GSE) were tested against peri-implantitis microflora. Suspension of microorganisms was made in sterile normal saline and adjusted to 0.5 Macfarland standard (10 8  Cfu mL -1 ). From the stock of 65536 mg mL -1  GSEs, serial dilutions were made up to 4 mg mL -1 . It was found that, no effective restriction and/or inhibition for tested fungi and Gram-negative  Escherichia coli 35218,  Klebsiella pneumonia  700603,  Pseudomonas aeruginosa  27853, and Gram-positive  Enterococcus feacalis 51299 bacteria while Gram-positive  Staphylococus aureu  44300 was inhibited at 32768 μg mL -1 GSE of ‘Muskule’ variety and GSE 65536 μg mL -1 GSE of ‘Okuzgozu’ and  Streptococcus pneumonia  49619 bacteria were inhibited at 2048 μg mL -1 4096 μg mL -1 and 32768 μg mL -1 concentration of ‘Kara Dimrit’, ‘Okuzgozu’ and ‘Muskule’ GSEs. The results of the study showed that GSEs has potential antimicrobial effects which can be further studied.

Effectiveness of Grape (Vitis Vinifera L.) Seed Extracts on Fungi and Bacteria Management