Possible roles of insulin and insulin-like growth factors in rat preimplantation development: investigation of gene expression by reverse transcription-polymerase chain reaction | Zendy

Xiao Zhang | Zendy; Gerald M. Kidder | Zendy; Andrew J. Watson | Zendy; Gilbert A. Schultz | Zendy; David T. Armstrong | Zendy

Open Access

Possible roles of insulin and insulin-like growth factors in rat preimplantation development: investigation of gene expression by reverse transcription-polymerase chain reaction

Author(s) -

Xiao Zhang,

Gerald M. Kidder,

Andrew J. Watson,

Gilbert A. Schultz,

David T. Armstrong

Publication year - 1994

Publication title -

reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.208

H-Index - 136

eISSN - 1741-7899

pISSN - 1470-1626

DOI - 10.1530/jrf.0.1000375

Subject(s) - autocrine signalling , embryo , paracrine signalling , insulin , biology , messenger rna , reverse transcription polymerase chain reaction , endocrinology , medicine , receptor , insulin like growth factor 2 , insulin like growth factor , gene expression , reverse transcriptase , endocrine system , andrology , hormone , microbiology and biotechnology , polymerase chain reaction , growth factor , gene , genetics

The sensitive mRNA phenotyping technique of reverse transcription-polymerase chain reaction was used to demonstrate that insulin receptor mRNA is present in rat embryos during the preimplantation period. In addition, mRNA encoding insulin-like growth factor (IGF) type I and type II receptors have also been detected in rat preimplantation embryos. IGF-I mRNA was not detected in preimplantation embryos but was found in oviducts and uteri of prepubertal and early pregnant rats. IGF-II mRNA was present in both embryos and in oviducts and uteri during the preimplantation period. These findings suggest that insulin and IGF-I could influence early embryo development in endocrine or in paracrine fashions, whereas IGF-II may have an additional autocrine mode of action in affecting preimplantation embryos in rats.

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