Open AccessEffect of amphotericin B and gestational age on sodium transport across the rat visceral yolk sac placenta in vitro
Author(s) -
John S. Gibson,
J. Clive Ellory
Publication year - 1984
Publication title -
reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.208
H-Index - 136
eISSN - 1741-7899
pISSN - 1470-1626
DOI - 10.1530/jrf.0.0720529
Subject(s) - yolk sac , fetus , placenta , gestation , basal (medicine) , gestational age , conceptus , andrology , endocrinology , medicine , chemistry , pregnancy , biology , embryo , microbiology and biotechnology , genetics , insulin
The transport properties of the rat visceral yolk sac placenta from Days 14.5 to 18.5 of gestation were studied in vitro. All tissues had a positive potential difference, fetal side relative to maternal side, and showed net Na transport towards the fetus. Basal short-circuit current and net Na flux increased rapidly with gestational age over the period studied. Amphotericin B applied to the maternal surface of the yolk sac stimulated current and net Na flux, indicating that the apical membrane Na permeability limited transport and revealing a reserve capacity for transport. Contrary to their basal values, current and Na flux following treatment with amphotericin were independent of gestational age.