Enzyme-immunoassay for determination of progesterone in milk from cows | Zendy

K.I. Arnstadt | Zendy; William F. Cleere | Zendy

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Enzyme-immunoassay for determination of progesterone in milk from cows

Author(s) -

K.I. Arnstadt,

William F. Cleere

Publication year - 1981

Publication title -

reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.208

H-Index - 136

eISSN - 1741-7899

pISSN - 1470-1626

DOI - 10.1530/jrf.0.0620173

Subject(s) - immunoassay , horseradish peroxidase , estrous cycle , chemistry , peroxidase , enzyme , chromatography , pregnancy , endocrinology , andrology , medicine , zoology , biology , biochemistry , antibody , immunology , genetics

An enzyme-immunoassay using horseradish peroxidase as label is described. The assay has a sensitivity of 0.1-20 pmol, i.e. 0.03-0.63 ng and intra- and inter-assay coefficients of variation of less than 10% when used for the determination of progesterone in samples of milk from cows. Comparison with a radio-immunoassay based on tritiated tracer showed excellent correlation (r = 0.98) for milk samples from cows during the oestrous cycle and early pregnancy.

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