FACTORS AFFECTING THE DILUTION, FREEZING AND STORAGE OF HUMAN SEMEN | Zendy

M. Freund | Zendy; Joseph Wiederman | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

FACTORS AFFECTING THE DILUTION, FREEZING AND STORAGE OF HUMAN SEMEN

Author(s) -

M. Freund,

Joseph Wiederman

Publication year - 1966

Publication title -

reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.208

H-Index - 136

eISSN - 1741-7899

pISSN - 1470-1626

DOI - 10.1530/jrf.0.0110001

Subject(s) - diluent , semen , motility , dilution , cryopreservation , andrology , liquid nitrogen , glycerol , chemistry , zoology , sperm motility , biology , biochemistry , medicine , nuclear chemistry , embryo , physics , organic chemistry , microbiology and biotechnology , thermodynamics , genetics

The presence of a true `dilution effect', as opposed to the toxic effects of certain diluents, was indicated in experiments where human semen was diluted 1:1, 1:5, 1 : 25 and 1 : 125 with six different diluents. Norman-Johnson-Solutions 1 and 2 were found to be superior to all other diluents tested and were shown to be equally useful as media in the freezing of diluted human semen. Twenty-one freezing trials were made, using the technique as developed to this point\p=m-\ 1 : 5 dilution with Norman-Johnson-Solutions 1 or 2, the addition of 10% egg yolk, slow cooling from +25 to +5\s=deg\C over a 4-hr period, 6 hr of equilibration with 7% glycerol at +5\s=deg\C, slow freezing from +5 to -70\s=deg\C at the rate of -1\s=deg\C/min, and storage at temperatures of

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research