IMMUNOCHEMICAL AND ELECTROPHORETIC PROPERTIES OF OESTROUS RABBIT UTERINE FLUID PROTEINS OBTAINED BY UTERINE LIGATION | Zendy

K. R. STEVENS | Zendy; H. D. Hafs | Zendy; A.G. Hunter | Zendy

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IMMUNOCHEMICAL AND ELECTROPHORETIC PROPERTIES OF OESTROUS RABBIT UTERINE FLUID PROTEINS OBTAINED BY UTERINE LIGATION

Author(s) -

K. R. STEVENS,

H. D. Hafs,

A.G. Hunter

Publication year - 1964

Publication title -

reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.208

H-Index - 136

eISSN - 1741-7899

pISSN - 1470-1626

DOI - 10.1530/jrf.0.0080319

Subject(s) - immunoelectrophoresis , electrophoresis , albumin , blood proteins , antiserum , precipitin , chemistry , antigen , microbiology and biotechnology , chromatography , biology , immunology , biochemistry

Fluids were obtained from 185 rabbit uteri 1 to 20 weeks after uterine ligation. The uterine fluid proteins were concentrated with an ultrafilter and characterized by diffusion in agar gel, moving boundary electrophoresis, and immunoelectrophoresis. Eight electrophoretic components were identified by means of moving boundary electrophoresis. One which migrated as a pre-albumin and another which migrated as an alpha-globulin were not found in blood sera. Agar-gel diffusion tests revealed thirteen antigenic components in uterine fluid. Three precipitin lines appeared to be specific to uterine fluid after absorption ofguinea-pig antisera to rabbit uterine fluid with rabbit blood

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