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Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc
Author(s) -
Nellas Ioannis,
Iyer K Venkatesan,
IglesiasArtola Juan M,
Pippel Martin,
Nadler André,
Eaton Suzanne,
Dye Natalie A
Publication year - 2022
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202154025
Subject(s) - microbiology and biotechnology , glycolysis , drosophila (subgenus) , wing , biology , production (economics) , signal transduction , chemistry , biochemistry , metabolism , gene , physics , macroeconomics , economics , thermodynamics
Adenosine triphosphate (ATP) production and utilization is critically important for animal development. How these processes are regulated in space and time during tissue growth remains largely unclear. We used a FRET‐based sensor to dynamically monitor ATP levels across a growing tissue, using the Drosophila larval wing disc. Although steady‐state levels of ATP are spatially uniform across the wing pouch, inhibiting oxidative phosphorylation reveals spatial differences in metabolic behavior, whereby signaling centers at compartment boundaries produce more ATP from glycolysis than the rest of the tissue. Genetic perturbations indicate that the conserved Hedgehog signaling pathway can enhance ATP production by glycolysis. Collectively, our work suggests the existence of a homeostatic feedback loop between Hh signaling and glycolysis, advancing our understanding of the connection between conserved developmental patterning genes and ATP production during animal tissue development.