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Deubiquitinating enzymes UBP12 and UBP13 stabilize the brassinosteroid receptor BRI1
Author(s) -
Luo Yongming,
Takagi Junpei,
Claus Lucas Alves Neubus,
Zhang Chao,
Yasuda Shigetaka,
Hasegawa Yoko,
Yamaguchi Junji,
Shan Libo,
Russinova Eugenia,
Sato Takeo
Publication year - 2022
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.15252/embr.202153354
Subject(s) - deubiquitinating enzyme , brassinosteroid , enzyme , receptor , microbiology and biotechnology , chemistry , biology , ubiquitin , biochemistry , gene , arabidopsis , mutant
Protein ubiquitination is a dynamic and reversible post‐translational modification that controls diverse cellular processes in eukaryotes. Ubiquitin‐dependent internalization, recycling, and degradation are important mechanisms that regulate the activity and the abundance of plasma membrane (PM)‐localized proteins. In plants, although several ubiquitin ligases are implicated in these processes, no deubiquitinating enzymes (DUBs), have been identified that directly remove ubiquitin from membrane proteins and limit their vacuolar degradation. Here, we discover two DUB proteins, UBP12 and UBP13, that directly target the PM‐localized brassinosteroid (BR) receptor BR INSENSITIVE1 (BRI1) in Arabidopsis . BRI1 protein abundance is decreased in the ubp1 2 i / ubp13 double mutant that displayed severe growth defects and reduced sensitivity to BRs. UBP13 directly interacts with and effectively removes K63‐linked polyubiquitin chains from BRI1, thereby negatively modulating its vacuolar targeting and degradation. Our study reveals that UBP12 and UBP13 play crucial roles in governing BRI1 abundance and BR signaling activity to regulate plant growth.