Genotype Characterization of Human Hydatid Cyst Isolates From Patients in Karaj, Iran, Using COX1 Gene Sequence | Zendy

Maryam Salimi | Zendy; Abolfazl Miahipour | Zendy; Mohammad Zibaei | Zendy; Sasan Rezaie | Zendy

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Genotype Characterization of Human Hydatid Cyst Isolates From Patients in Karaj, Iran, Using COX1 Gene Sequence

Author(s) -

Maryam Salimi,

Abolfazl Miahipour,

Mohammad Zibaei,

Sasan Rezaie

Publication year - 2019

Publication title -

international journal of enteric pathogens

Language(s) - English

Resource type - Journals

eISSN - 2345-3362

pISSN - 2322-5866

DOI - 10.15171/ijep.2019.07

Subject(s) - echinococcus granulosus , polymerase chain reaction , genotype , hydatid cyst , shahid , echinococcosis , cyst , biology , veterinary medicine , cystic echinococcosis , gene , pathology , medicine , microbiology and biotechnology , genetics , zoology , philosophy , theology

Background: Cystic echinococcosis is a main zoonotic infection. It can cause serious clinical problems for human health around the world. Genotypic specification of Echinococcus granulosus in human is important due to control and prevention programs. Objective: In this investigation, genetic characteristics of human isolates of E. granulosus in Karaj, Iran, were studied. Materials and Methods: In this review, 3 isolates of surgically removed hydatid cysts were obtained from patients in Shahid Madani hospital, Karaj, Iran in 2014. DNA was extracted from the protoscolex of the cyst, and polymerase chain reaction (PCR) assay was done on the COX1 gene. Results: DNA fragments were sequenced and the results were aligned and analyzed. Among the isolates, 3 (100%) were E. granulosus (G1) strain. Conclusion: The G1 genotype was the most superior strain from human isolates of hydatid cyst in Karaj.

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