Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey | Zendy

Davut Bayram | Zendy

Open Access

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Author(s) -

Davut Bayram

Publication year - 2017

Publication title -

ankara üniversitesi veteriner fakültesi dergisi

Language(s) - Turkish

Resource type - Journals

SCImago Journal Rank - 0.184

H-Index - 12

eISSN - 1308-2817

pISSN - 1300-0861

DOI - 10.1501/vetfak_0000002818

Subject(s) - restriction fragment length polymorphism , biology , allele , gene , genetics , microbiology and biotechnology , polymorphism (computer science) , polymerase chain reaction , allele frequency , breed

Bu calismada Turkiye’de Kayseri civarinda yetistirilen Holstayn irki sigirlarda (n=352) leptin (LEP) ve hipofiz bezine ozgu transkripsiyon faktoru -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaclanmistir. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amaci ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parca uzunluk polimorfizm (RFLP) analizi yapilmistir. Pit-1 geni icin 600 bc’lik ve LEP geni icin 422 bc’lik uzunluk amplifiye edilmistir. Calismada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak uzere iki iki tip allel gozlendi. En yuksek frekans Pit-1 geni icin A allelinde (0.87), LEP geni icin ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuclarina gore, incelenen irkta, her iki gende de Hardy-Weinberg dengesinden anlamli bir sapma gozlendi (ayni sira ile; X2:40.75, p<0.001ve X2:8.4, p<0.01)

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