Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish native cattle breeds

The aim of this study was to identify whether there is any mutant allele of Bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID) in Turkish native cattle breeds or not. These genetic disorders are autosomal recessive hereditary disorders which causes significant economic loses on dairy cattle breeding worldwide. In this study, 200 Anatolian Black, 100 East Anatolian Red, 100 Turkish Grey, 50 Anatolian Southern Yellow, 50 South Anatolian Red and 9 Zavot cattle (total 509 Turkish native cattle) were screened in order to identify BLAD, DUMPS, CVM, BC and FXID genotypes. Genomic DNA was obtained from blood and the amplicons were obtained by using PCR. PCR products were digested with Taq I, Ava I, Eco T22I and Ava II restriction enzymes for BLAD, DUMPS, CVM, and BC, respectively. Digested products and PCR product of FXID were analyzed by agarose gel electrophoresis stained with ethidium bromide. The results of this study demonstrated that there was no carrier for these genetic disorders in 509 Turkish native cattle examined. According to the results of this study, it can be clearly said that mutant allele of BLAD, DUMPS, CVM, BC and FXID is absent in Turkish native cattle breeds.